2017 Fiscal Year Final Research Report
Exploration and functional analysis of lncRNA associated with pituitary tumorigenesis
| Project/Area Number |
15K09432
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Endocrinology
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| Research Institution | Kobe University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
高橋 裕 神戸大学, 医学研究科, 准教授 (70301281)
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| Co-Investigator(Renkei-kenkyūsha) |
YAMADA Shozo 虎の門病院, 間脳下垂体腫瘍外科 (80260131)
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| Research Collaborator |
YOSHIDA Kenichi
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| Project Period (FY) |
2015-04-01 – 2018-03-31
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| Keywords | Cushing disease / long non-coding RNA / proliferation / invasiveness / ACTH |
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| Outline of Final Research Achievements |
In this research, we explored long non-coding RNA (lncRNA) which associated with proliferation and invasiveness in ACTH producing pituitary adenoma (ACTHoma). First, we divided specimens of ACTHoma from 7 patients according to Knosp grade; grade <2, and grade 2 and more, and compared their lncRNA expression levels using microarray analysis. Furthermore, we measured RNA levels of 30 ACTHoma specimens and compared with their clinical characterization. Then we found lncRNA CRNDE, which expressed 3.6 fold in tumors with grade 2 and more.
CRNDE expressed 3 fold in macroadenoma than in microadenoma, suggesting this lncRNA is associated with tumor proliferation in addition to invasiveness. CRNDE located in cytoplasm of ACTHoma, indicating the mechanism related to proliferation and invasiveness by CRNDE is mediated via microRNA or mRNA stability as an epigenetic regulation. Further molecular mechanism needs to be analyzed of CRNDE in ACTHoma as a potential therapeutic target.
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| Free Research Field |
内分泌内科
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