2017 Fiscal Year Final Research Report
Establishment of in vivo model of anemia by shRNA transgene controlled by erythroid-lineage specific promoter
| Project/Area Number |
15K09447
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Hematology
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| Research Institution | Tohoku University |
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Principal Investigator |
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| Project Period (FY) |
2015-04-01 – 2018-03-31
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| Keywords | 血液内科学 / 貧血 |
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| Outline of Final Research Achievements |
To establish in vivo model of anemia, we established and crossed 1) GATA-1 promoter (G1HRD)/reverse tetracycline transactivator (rtTA)-transgenic mice and 2) Tetracycline-responsive element (TRE)/Lmo2 shRNA-tranegenic mice. In the established mice (Tg-TRE-Lmo2 shRNA::Tg-G1HRD-rtTA), Lmo2 shRNA is expected to be induced specifically in erythroblasts by tetracycline administration, leading to the onset of anemia. Our established system would be applied to establish wide variety of in vivo anemia models by targeting to erythroid genes other than Lmo2.
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| Free Research Field |
医歯薬学
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