2017 Fiscal Year Final Research Report
Development of transgenic T lymphocytes to break down the immunosuppressive tumor microenvironment and its functional analysis
| Project/Area Number |
15K09497
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Hematology
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| Research Institution | Nagoya University |
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Principal Investigator |
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| Research Collaborator |
OKUNO Shingo
JULAMANEE Jakrawadee
MIYAO Kotaro
SAKEMURA Reona
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| Project Period (FY) |
2015-04-01 – 2018-03-31
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| Keywords | キメラ抗原レセプター / 遺伝子導入T細胞療法 / CD8陽性細胞 / Tリンパ球 / 腫瘍微小環境 |
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| Outline of Final Research Achievements |
Based on the amino acid sequence information of the antibody against the molecule PD-L1 thought to be expressed on tumor cells in order that tumor cells escape from immune cells, a single-chain antibody binding to PD-L1 was prepared. We further created a novel molecule with CD28 or 4-1BB or both as a part of domain. When this antibody was stained by fluorescent-labeled PD-L1 molecule, it was found that it stained well, indicating that it binds well to the antigen. This gene was transfected into T lymphocytes and its function was examined in vitro and in vivo in mice. On the other hand, we tried to knock out CTLA-4 molecule, which is expressed after activation of T cells and supposedly acts suppressively on self.
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| Free Research Field |
遺伝子導入T細胞療法
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