Analysis of bronchial asthma onset/exacerbation mechanism based on inflammatory dendritic cells and basophils.

2017 Fiscal Year Final Research Report

• Project/Area Number: 15K09549
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Collagenous pathology/Allergology
• Research Institution: Nagoya University
• Principal Investigator: Wakahara Keiko 名古屋大学, 医学部附属病院, 講師 (00631433)
• Research Collaborator: SUZUKI Yoshihiro ; NISHIO Tomoko
• Project Period (FY): 2015-04-01 – 2018-03-31
• Keywords: 気管支喘息 / 好塩基球 / 炎症性樹状細胞

Outline of Final Research Achievements

To analyze the role of inflammatory dendritic cells and basophils as bronchial asthma onset and exacerbation factors, we performed following studies using induced sputum from asthmatic patients. 1) Establishment of induced sputum analysis by flow cytometry, 2) Analysis of inflammatory dendritic cells and basophils in induced sputum, 3) Phenotype identification of sputum basophils, 4) Relationship between sputum basophils and clinical phenotype in adult asthma.
The numbers of inflammatory dendritic cells and basophils in induced sputum were increased in adult asthmatic patients compared with those in normal controls. The number of sputum basophils was positively correlated with the number of sputum eosinophils. Furthermore, sputum basophils were activated compared with blood basophils from same patients. These observations suggest that basophils could be exacerbation factors of eosinophilic asthma.

Free Research Field

呼吸器学、アレルギー学、免疫学