Functional analysis of PRSS33, a eosinophil-specific protease

2017 Fiscal Year Final Research Report

• Project/Area Number: 15K09560
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Collagenous pathology/Allergology
• Research Institution: National Center for Child Health and Development
• Principal Investigator: Matsumoro Kenji 国立研究開発法人国立成育医療研究センター, 免疫アレルギー・感染研究部, 部長 (60181765)
• Co-Investigator(Renkei-kenkyūsha): Toyama Sumika 国立成育医療研究センター研究所, 免疫アレルギー・感染研究部 (50805893)
• Project Period (FY): 2015-04-01 – 2018-03-31
• Keywords: 好酸球 / プロテアーゼ / PRSS33

Outline of Final Research Achievements

We investigated the function of an eosinophil-specific proteases/proteinase protease serine 33 (PRSS33), in vitro and in vivo. GM-CSF-activated human eosinophils but not resting eosinophils were stained with anti-PRSS33 mAb, suggesting that PRSS33 is specifically expressed on the granular membrane of eosinophils. However, PRSS33 was not secreted to the supernatant even after stimulation with secretagogues. Recombinant PRSS33 protein (rPRSS33) created with a baculovirus system induced significant expression of collagen and fibronectin mRNAs in human fibroblasts at least in part via protease-activated receptor-2 activation. PRSS33 knockout mouse created by CRISPR/Cas9 system was employed to papain-inhalation model and long term-house dust mite-inhalation model, however, no significant effects were observed.

Free Research Field

免疫、アレルギー学