Study of the protective immune mechanism against tuberculosis through pDC activation induced by CpG-ODN and Mycobacterium tuberculosis antigen

2017 Fiscal Year Final Research Report

• Project/Area Number: 15K09565
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Infectious disease medicine
• Research Institution: University of Fukui
• Principal Investigator: Suzuki Fumiko 福井大学, 学術研究院医学系部門, 特命助教 (80291376)
• Co-Investigator(Kenkyū-buntansha): 島田 一郎 福井大学, 学術研究院医学系部門, 教授 (20272908) ; 西山 晃史 新潟大学, 医歯学系, 講師 (80452069)
• Co-Investigator(Renkei-kenkyūsha): MATSUMOTO Sohkichi 新潟大学, 医歯学系, 教授 (30244073)
• Project Period (FY): 2015-04-01 – 2018-03-31
• Keywords: 結核 / CpGオリゴDNA / 形質細胞様樹状細胞 / 骨髄系樹状細胞 / 共刺激分子

Outline of Final Research Achievements

The major protein of Mycobacterium tuberculosis, “M”, is expressed both in the growth phase and in the resting phase. On the other hand, the palindromic deoxyoligonucleotide, “G”, containing a CpG motif induces the activation of plasmacytoid dendritic cells (pDCs). Human peripheral mononuclear cells co-cultured with a combination of “M” and “G” showed enhanced IFN-α production from the pDCs and upregulation of costimulators on the surface of dendritic cells (DCs). It is suggested that both increases in IFN-α production and costimulator expression are dependent on efficient transfer of “G” to DCs mediated by “M”.
The synergistic immune mechanism induced by the combination of “M” antigen and “G” adjuvant is valuable as the scientific basis for the development of new vaccines against tuberculosis.

Free Research Field

分子免疫