2017 Fiscal Year Final Research Report
Proteomic analysis for drug-resistant mechanisms of Staphylococcus aureus
| Project/Area Number |
15K09586
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Infectious disease medicine
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| Research Institution | Showa Pharmaceutical University (2017)
St. Marianna University School of Medicine (2015-2016) |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
浅井 大輔 聖マリアンナ医科大学, 医学部, 講師 (10423485)
有戸 光美 聖マリアンナ医科大学, 医学部, 講師 (00509911)
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| Project Period (FY) |
2015-04-01 – 2018-03-31
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| Keywords | 黄色ブドウ球菌 / 薬剤耐性 / プロテオミクス |
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| Outline of Final Research Achievements |
Drug-resistant bacteria are great concern in medicine around the world. To clarify the precise mechanism of the resistance, we attempted to establish de novo method which can analyze the bacterial proteomics efficiently and precisely. A conventional method of 2D-PAGE analysis revealed that the expression level of 46 proteins in methicillin-resistant Staphylococcus aureus (MRSA) varied significantly under the cultivation with high antibiotics (oxacillin) concentration. We are constructing a recombinant protein library of MRSA for the internal control of the de novo proteomic analysis.
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| Free Research Field |
微生物学
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