2017 Fiscal Year Final Research Report
Identifying epigenetic mechanisms for testicular development and developing innovative technology for regenerative medicine
| Project/Area Number |
15K09615
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Pediatrics
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| Research Institution | Tokyo Medical and Dental University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
森尾 友宏 東京医科歯科大学, 大学院医歯学総合研究科, 教授 (30239628)
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| Project Period (FY) |
2015-04-01 – 2018-03-31
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| Keywords | 性分化 / エピジェネティックス / 精巣 / CRSPR/Cas9 / シトルリン化 |
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| Outline of Final Research Achievements |
In this study, we aimed to clarify epigenetic mechanisms in development of the testicular supporting cells, Sertoli cells.
Peptidylarginine deiminases (PADs) are a family of enzymes that change the charge of target proteins through citrullination. We identified Padi2 which encodes PAD2 was exclusively expressed in Sertoli cells of fetal developing testes. Our data suggested that Padi2 was a novel gene in fetal testis development which was specifically expressed in Sertoli cells through the regulation of SOX9 and FOXL2 and supported regulation by SOX9 for target genes. The phenotype of Padi2(-/-)XY indicates that a redundant factor compensates for the role of PAD2 in testicular development.
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| Free Research Field |
小児内分泌
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