Effects of antibiotic exposure during early infant on mucosal innate lymphoid cells and their relation to bronchial asthma

2017 Fiscal Year Final Research Report

• Project/Area Number: 15K09668
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Pediatrics
• Research Institution: Nippon Medical School
• Principal Investigator: Yamanishi Shingo 日本医科大学, 医学部, 助教 (00465337)
• Co-Investigator(Kenkyū-buntansha): Ruby Pawankar 日本医科大学, 医学部, 助教授 (00287674) ; 田辺 雄次郎 日本医科大学, 医学部, 助教 (00643818) ; 尾崎 優介 日本医科大学, 医学部, 助教 (60740561)
• Project Period (FY): 2015-04-01 – 2018-03-31
• Keywords: 腸内細菌叢 / 粘膜免疫 / 自然リンパ球 / 気管支喘息 / 乳児早期 / 抗菌薬

Outline of Final Research Achievements

We examined whether changes in mucosal immune cell profile, especially a profile of innate lymphoid cells, through altering the intestinal microbiota caused by antibiotic exposure, are associated with pathophysiology of asthma. First, we examined the effects of antibiotic exposure using Amoxicillin during infant on murine intestinal microbiota, we found that Amoxicillin exposure resulted in predominance of genus Bacteroides, with disappearance of genus Lactobacillus and genus Bifidobacterium. Also, we found decreased intestinal microbial diversity in Amoxicillin group compared with control group. Next, we examined mucosal immune cell profile in both intestine and lung, we found that profound decrease in mucosal immune cells and ILC2 cells in the intestine.

Free Research Field

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