Analysis of the onset and curative mechanism of food allergy using multicolor flow cytometry.

2017 Fiscal Year Final Research Report

• Project/Area Number: 15K09669
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Pediatrics
• Research Institution: Fujita Health University
• Principal Investigator: Tsuge Ikuya 藤田保健衛生大学, 医学部, 教授 (00231431)
• Project Period (FY): 2015-04-01 – 2018-03-31
• Keywords: 食物アレルギー / 経口免疫療法 / アレルゲン特異的T細胞 / 好塩基球 / フローサイトメトリー

Outline of Final Research Achievements

This study aimed to analyze the dynamics of allergen-specific type 2 helper T cells (Th2), regulatory T cells (Treg) and basophils in oral immunotherapy (OIT) participants to improve the treatment of food allergies.
After 4 months, patients receiving OIT using hypoallergenic peptide milk exhibited a marked decline in the number of IL-4 producing milk-specific T cells, implying OIT exerts its effects through the inhibition of Th2. In OIT combined with anti-IgE antibody therapy, the basophil activation test (BAT) was completely depressed by anti-IgE therapy only in a case where the succeeding OIT was effective. It might suggest that BAT is useful for determining the optimal dose of anti-IgE antibody and predicting its efficacy.

Free Research Field

小児科学