Trim32-based new differentiation therapy for neuroblastoma

2017 Fiscal Year Final Research Report

• Project/Area Number: 15K09674
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Pediatrics
• Research Institution: 地方独立行政法人佐賀県医療センター好生館(ライフサイエンス研究所)
• Principal Investigator: IZUMI Hideki 地方独立行政法人佐賀県医療センター好生館(ライフサイエンス研究所), ライフサイエンス研究所, 部長 (10397987)
• Project Period (FY): 2015-04-01 – 2018-03-31
• Keywords: 分化誘導療法 / 神経芽腫

Outline of Final Research Achievements

The purpose of this study is to clarify the molecular target of Trim32-based differentiation therapy for neuroblastoma cells and to establish a new treatment method of neuroblastoma by a novel mechanism. We attempted to identify new target gene (s) in Trim32-mediated cell differentiation by cDNA microarray. As a result of analysis by cDNA microarray, CDKN1A was identified as a gene whose expression increased in Trim32-mediated cell differentiation. When CDKN1A is knocked down in human neuroblastoma cells, 13-cis-retinoid (13-cis) mediated cell differentiation ability decreased. Furthermore, when 13-cis treatment is performed on human neuroblastoma cells after transfection of CDKN1A cDNA expression vector, the differentiation inducing ability significantly increased.

Free Research Field

小児科学