A study of heat shock proteins in constriction and relaxation response to oxygen stimuli in the ductus arteriosus

2017 Fiscal Year Final Research Report

• Project/Area Number: 15K09730
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Embryonic/Neonatal medicine
• Research Institution: Tokyo Women's Medical University
• Principal Investigator: Hayama Emiko 東京女子医科大学, 医学部, 非常勤講師 (00349698)
• Co-Investigator(Renkei-kenkyūsha): NAKANISHI Toshio 東京女子医科大学, 医学部, 特任教授 (90120013)
• Project Period (FY): 2015-04-01 – 2018-03-31
• Keywords: 動脈管 / ヒートショックタンパク質 / 酸素感受性

Outline of Final Research Achievements

Specific expression of Hsp72 (HspA1B) in the near term ductus arteriosus (DA) was confirmed in both rabbit and rat. Expression of Hsp72 and apoptotic cells in newborn rabbit DA media was separated near adventitia and near lumen respectively. Identical expression pattern of HspA1B and c-Fos mRNA in fetal and newborn DA, the adjacent aorta (Ao) and pulmonary artery (PA) was detected. c-FOS plays a regulatory role in growth, differentiation and apoptosis. c-FOS proteins do not dimerize with each other and only bind to DNA as AP-1 transcription factor when bound with c-JUN. However, c-Jun was expressed relatively lower in the DA than the Ao and PA, suggesting existence of different binding partners. Expression of several stress response gene mRNAs related c-Fos was determined in the mature fetal DA with oxygen stimuli.

Free Research Field

分子生物学