Molecular mechanism by which circadian rhythm affects development and refractory of allergic disorders.

2017 Fiscal Year Final Research Report

• Project/Area Number: 15K09776
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Dermatology
• Research Institution: Kyoto Prefectural University of Medicine
• Principal Investigator: KATOH NORITO 京都府立医科大学, 医学(系)研究科(研究院), 教授 (30244578)
• Co-Investigator(Kenkyū-buntansha): 峠岡 理沙 京都府立医科大学, 医学(系)研究科(研究院), 講師 (80464585) ; 八木田 和弘 京都府立医科大学, 医学(系)研究科(研究院), 教授 (90324920)
• Project Period (FY): 2015-04-01 – 2018-03-31
• Keywords: アレルギー

Outline of Final Research Achievements

The purpose of this study was to investigate the effects of circadian rhythm disruption on the development of immune tolerance by the perturbation of light environment. Mice were kept under constant light (LL) or light-dark (LD) conditions, and hapten was applied at 2days after birth. Six weeks later, hapten was reapplied to abdominal skin, followed by hapten application to ear skin 5days later. The ear-swelling responses and cell infiltration into inflamed skin significantly increased in LL mice compared with those in LD mice. The percentage and the number of Foxp3+-regulatory T cells notably decreased in inflamed skin and draining lymph nodes of LL mice compared with that in LD mice. Loss-of-function mutation of a key circadian gene, Bmal1, also exacerbated the ear-swelling responses and cell infiltration into inflamed skin in mice. These results suggest that circadian rhythm may be implicated in immune tolerance development in allergic inflammation.

Free Research Field

皮膚科学