Establishment of AD model mice harboring novel pathogenic PS1 mutation presenting "Behavioral and Psychological Symptoms of Dementia"

2017 Fiscal Year Final Research Report

• Project/Area Number: 15K09840
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Psychiatric science
• Research Institution: Sapporo Medical University
• Principal Investigator: Kawamata Jun 札幌医科大学, 医学部, 准教授 (60360814)
• Co-Investigator(Kenkyū-buntansha): 下濱 俊 札幌医科大学, 医学部, 教授 (60235687)
• Project Period (FY): 2015-04-01 – 2018-03-31
• Keywords: アルツハイマー病 / BPSD / プレセニリン１ / トランスジェニックマウス / CRISPR/Cas9

Outline of Final Research Achievements

We report the familial Alzheimer pedigree carrying a novel mutation, three bases deletion, which results in one amino acid deletion of PS1 (Psen1 delta p.277). To establish the adequate animal model of AD especially presenting BPSD-like symptoms, we planned to make the transgenic mice harboring the mutation using CRISPR/Cas9 method. Even after injecting more than 200 times, no living mutant mouse was obtained. Remodeling guide RNA(crRNA) results in successfully establishing the transgenic mice, which will enable the neuropathological and behavioral analysis after aging of these transgenic mice, both heterozygote and possibly homozygote.

Free Research Field

臨床神経学