2017 Fiscal Year Final Research Report
Clinical application of candidate stem cell markers for detection of circulating tumor cells in esophageal cancer.
| Project/Area Number |
15K10088
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Digestive surgery
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| Research Institution | University of Toyama |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
嶋田 裕 京都大学, 医学(系)研究科(研究院), 講師 (30216072)
塚田 一博 富山大学, 大学院医学薬学研究部(医学), 教授 (90171967)
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| Project Period (FY) |
2015-04-01 – 2018-03-31
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| Keywords | cancer stem cells / esophageal cancer / circulating tumor cells |
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| Outline of Final Research Achievements |
We isolated and characterized quiescent cancer stem cells (CSCs) population in esophageal squamous cell carcinoma (ESCC). We isolated p75NTR-positive cells in the G0-G1 phase but not in the S-G2-M phase showed strong expression of stem cell-related genes; high colony formation ability; high tumorigenicity in a mouse xenograft model; and strong chemoresistance, suggesting that p75NTR-positive/G0-1 cells represent quiescent CSCs in ESCC. For diagnostic application , our two-color flow cytometric investigation using peripheral blood samples of ESCC patients revealed that p75NTR-positive, but not p75NTR-negative circulating tumor stem cell counts, correlated with clinically diagnosed distant metastasis and pathological venous invasion in ESCC tumors. Further investigation targetting CSCs which are specifically characterized by multiple markers may provide us with novel therapeutic and diagnostic strategies for patients with ESCC.
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| Free Research Field |
消化器外科学
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