Development of the new treatment strategy targeting microRNA which controls histone de-methylase

2017 Fiscal Year Final Research Report

• Project/Area Number: 15K10142
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Digestive surgery
• Research Institution: Kumamoto University
• Principal Investigator: SAKAMOTO Yasuo 熊本大学, 医学部附属病院, 非常勤診療医師 (00452897)
• Co-Investigator(Kenkyū-buntansha): 馬場 祥史 熊本大学, 医学部附属病院, 特任講師 (20599708) ; 宮本 裕士 熊本大学, 医学部附属病院, 講師 (80551259)
• Project Period (FY): 2015-04-01 – 2018-03-31
• Keywords: JMJD3 / microRNA / EZH2

Outline of Final Research Achievements

We identified microRNA, miR186-5p which suppress the expression of histone de-methylase JMJD3 with enzyme activity opposite to EZH2. We confirmed that the suppression of JMJD3, CDH1, VASH1, and TFAP2E by the over-expression of miR186-5p in colon cancer cell line. We confirmed that cisplatin sensitivity was increased when I controlled expression of histone methylase EZH2 in colon cancer cell line.

Free Research Field

消化器癌、DNAメチル化、ヒストンメチル化