Comprehensive diagnosis of bacterial infection associated with acute cholecystitis using metagenomic approach

2017 Fiscal Year Final Research Report

• Project/Area Number: 15K10198
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Digestive surgery
• Research Institution: Toho University
• Principal Investigator: ASAI Koji 東邦大学, 医学部, 講師 (20366454)
• Co-Investigator(Kenkyū-buntansha): 草地 信也 東邦大学, 医学部, 教授 (70169978)
• Co-Investigator(Renkei-kenkyūsha): KURODA Makoto 国立感染症研究所, 病原体ゲノム解析研究センター, センター長 (80317411) ; MATSUMOTO Tetsuya 東京医科大学, 医学部, 教授 (10256688) ; MAETANI Iruru 東邦大学, 医学部, 教授 (00219263) ; WATANABE Manabu 東邦大学, 医学部, 准教授 (30297709)
• Project Period (FY): 2015-04-01 – 2018-03-31
• Keywords: 急性胆道炎 / メタゲノム解析 / 抗菌薬感受性 / 耐性菌

Outline of Final Research Achievements

We undertook a metagenomic approach to characterize the bacterial infection associated with acute cholecystitis and cholangitis. More than 100 cases included this analyses during 3 years. Metagenomic analysis demonstrated possible single or multiple bacteria infections within 24 h. Also, antimicrobial resistance including extended-spectrum beta-lactamase gene could be identified in other 24 h. Metagenomic analysis was useful for rapid bacterial diagnosis including assessing potential antimicrobial susceptibility in patients with acute cholecystitis and cholangitis.
We have already reported preliminary 6 cases（Kujiraoka M, Front Microbial 2017）. Now, we prepare to report total analytic cases.

Free Research Field

消化器外科