2017 Fiscal Year Final Research Report
New approach in mechanisms of Tetralogy of Fallot with human iPS cells.
| Project/Area Number |
15K10222
|
|---|
| Research Category |
Grant-in-Aid for Scientific Research (C)
|
| Allocation Type | Multi-year Fund |
|---|
| Section | 一般 |
|---|
| Research Field |
Cardiovascular surgery
|
|---|
| Research Institution | Saitama Medical University |
|---|
Principal Investigator |
|
|---|
| Co-Investigator(Kenkyū-buntansha) |
千本松 孝明 埼玉医科大学, 医学部, 教授 (70216563)
|
|---|
| Project Period (FY) |
2015-04-01 – 2018-03-31
|
| Keywords | ファロー四徴症 / iPS細胞 |
|---|
| Outline of Final Research Achievements |
Using the thymus as remainder tissue after the surgery of Tetralogy of Fallot (TOF), fractional culture of fibroblasts was performed. The fibroblasts from the thymus had high potential to create human iPS cells (hiPSc) by various methods. Although Jarid2, Prdm14, Sall4a and Essrb1, which also have potential power to induce hiPSc, were used combined with OSKM that is the Yamanaka factors, there was no significant difference among them. Using this hiPSc, the cardiomyocytes differentiation was performed. The thymus derived fibroblasts had highly potential to develop the cardiomyocytes, however, at this time, it is still covered difference between TOF and normal.
|
| Free Research Field |
先天性心疾患
|
