Functional analysis of candidate genes for oncogenesis in thymic carcinoma and establishment of individualized therapy

2017 Fiscal Year Final Research Report

• Project/Area Number: 15K10279
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Respiratory surgery
• Research Institution: Nagoya City University
• Principal Investigator: Okuda Katsuhiro 名古屋市立大学, 大学院医学研究科, 准教授 (50529170)
• Co-Investigator(Kenkyū-buntansha): 矢野 智紀 名古屋市立大学, 大学院医学研究科, 研究員 (40315883) ; 森山 悟 名古屋市立大学, 大学院医学研究科, 研究員 (50551264)
• Project Period (FY): 2015-04-01 – 2018-03-31
• Keywords: ウイルスベクター / Gateway system / KIT / Tyrosine kinase domain / gene mutation / PD-L1

Outline of Final Research Achievements

We carried out functional analysis on 25 candidate genes of oncogenic driver mutation by using nest generation sequencer. Functional analysis was performed on KIT gene mutation, which is a potential candidate gene of oncogenic driver mutation, but it suggested that just only the KIT gene mutation may not have carcinogenicity. In addition, five tyrosine kinase domain mutations were analyzed for surgical cases of thymic carcinoma and lung cancer, but no mutation was observed.
We revealed that the positive cases of PD-L1 protein expression in thymic carcinoma is relatively high and immune checkpoint inhibitors can be an effective treatment for thymic carcinoma.

Free Research Field

呼吸器外科