Development of an anti-rupture drug treatment method using a novel animal model of easily rupturable large cerebral aneurysm and human clinical specimen

2017 Fiscal Year Final Research Report

• Project/Area Number: 15K10287
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Neurosurgery
• Research Institution: Hokkaido University
• Principal Investigator: NAKAYAMA Naoki 北海道大学, 医学研究院, 講師 (40421961)
• Co-Investigator(Kenkyū-buntansha): 森脇 拓也 北海道大学, 医学(系)研究科(研究院), 客員研究員 (30597464) ; 穂刈 正昭 北海道大学, 医学研究院, 客員研究員 (30622807) ; 鐙谷 武雄 北海道大学, 大学病院, 助教 (80270726) ; 数又 研 北海道大学, 大学病院, 講師 (60634144) ; 寳金 清博 北海道大学, 大学病院, 教授 (90229146)
• Research Collaborator: SHIMODA Yusuke 北海道大学, 医学研究院, 客員研究員
• Project Period (FY): 2015-04-01 – 2018-03-31
• Keywords: 脳動脈瘤 / 脳動脈瘤破裂 / 大型脳動脈瘤 / 動物モデル / 抗血小板剤

Outline of Final Research Achievements

In order to elucidate the rupture mechanism of the cerebral aneurysm, we first aimed to establish an animal model of easily rupturable large cerebral aneurysm. Hypertension was induced in male SD rats by ligation of the bilateral renal artery dorsal branch and high salinity, hemodynamic stress is given by left common carotid artery ligation, and the nitro compound was intraperitoneally administered as a stable fumaric acid compound every week. The dose of nitro compound was changed variously, aneurysm developed in about 80% of individuals at 1400 mg / kg, and large aneurysm was induced in 22%.
Furthermore, in order to raise the induction rate of large aneurysms, ovaries were removed in female rats simulating human postmenopausal women, in addition, the contralateral external carotid artery was ligated to enhance the hemodynamic stress. And we started experiments with medication by aspirin and cilostazol.

Free Research Field

脳血管障害