Protective effects of endogenous carbon monoxide on cerebral energy metabolism during acute focal brain ischemia

2017 Fiscal Year Final Research Report

• Project/Area Number: 15K10315
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Neurosurgery
• Research Institution: National Center for Global Health and Medicine (2016-2017); Keio University (2015)
• Principal Investigator: Takayuki Morikawa 国立研究開発法人国立国際医療研究センター, その他部局等, 研究員 (80465012)
• Project Period (FY): 2015-04-01 – 2018-03-31
• Keywords: 一酸化炭素

Outline of Final Research Achievements

Brain generates a micromolar order of carbon monoxide (CO) via heme oxygenase (HO) reactions. In this study, we examined the hypothesis that the deletion of HO-2 exacerbates cerebral energy metabolism during acute focal brain ischemia. We conducted quantitative imaging mass spectrometry (Q-IMS) analysis for adenylates and lactate to decipher local cerebral responses of energy metabolism from wild-type (WT) and HO-2-null mice that underwent a 60-min occlusion of the left middle cerebral artery. Striking differences were found in the contralateral hemispheres, where the levels of ADP, AMP and lactate in HO-2-null mice were higher than those in WT mice. These results suggest that the HO-2/CO system plays roles in protecting against ischemia not only at the ischemic core where the severe reduction of blood flow occurs, but it also protects even more effectively in the trans-hemispheric regions where only a subtle reduction of blood flow takes place.

Free Research Field

生体イメージング