Analysis of mechanisms to develop hydrocephalus using mice model

2017 Fiscal Year Final Research Report

• Project/Area Number: 15K10380
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Neurosurgery
• Research Institution: Chiba Cancer Center (Research Institute)
• Principal Investigator: Isogai Eriko 千葉県がんセンター(研究所), 発がん研究グループ 実験動物研究室, 上席研究員 (40300917)
• Co-Investigator(Kenkyū-buntansha): 若林 雄一 千葉県がんセンター(研究所), 発がん研究グループ 実験動物研究室, 室長 (40303119)
• Co-Investigator(Renkei-kenkyūsha): OHIRA Miki 千葉県がんセンター研究所, がんゲノム研究室, 室長 (20311384) ; HASEGAWA Yuzo 千葉県がんセンター研究所, 脳神経外科, 医師 (60436409)
• Project Period (FY): 2015-04-01 – 2018-03-31
• Keywords: 神経発生 / 水頭症 / モデル動物

Outline of Final Research Achievements

100 % of double transgenic mice (Tg) of Lmo3 and Hen2 developed hydrocephalus. The cortex, ventricular and subventricular zone were thin and intermediate progenitor cells (IPC) were increased in cortex of embryo. Over-expression of both of Lmo3 and Hen2 by in utero electroporation increased IPC but not neuron and each of them decreased IPC and increased neuron. Therefore, over-expression of each gene might decrease IPC and deplete stem cells by premature neurogenesis. On the other hand, over-expression of both genes might deplete stem cells by induction of excess IPC. Both cases could lead to aberrant neuronal development and hydrocephalus.

Free Research Field

neuronal development