Identification of novel microRNA and elucidation of tenocyte differentiation mechanism using tenocyte culture system

2017 Fiscal Year Final Research Report

• Project/Area Number: 15K10396
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Orthopaedic surgery
• Research Institution: Shinshu University
• Principal Investigator: Hayashi Masanori 信州大学, 学術研究院医学系, 助教 (20624703)
• Co-Investigator(Kenkyū-buntansha): 内山 茂晴 信州大学, 医学部, 特任教授 (10242679) ; 加藤 博之 信州大学, 学術研究院医学系, 教授 (40204490) ; 植村 一貴 信州大学, 医学部附属病院, 特任研究員 (50624706)
• Project Period (FY): 2015-04-01 – 2018-03-31
• Keywords: 腱細胞 / マイクロRNA

Outline of Final Research Achievements

Recently, microRNAs(miRNAs) have been emerged as important regulators in cell differentiation. However, miRNAs which regulate tenocyte differentiation have not been identified. In this study, we used a tenocyte culture system established in our previous study to identify and characterize new miRNAs that control tenocyte differentiation. Our study revealed that miR-675-3p, the expression of which decreases immediately after induction of differentiation, has the effect of promoting differentiation of tenocytes, suppressing the expression of Mef2a and Mef2c detected as its target gene. Our results suggest that miR-675-3p suppresses the expression of Mef2a and Mef2c, whereby controlling tenocyte differentiation.

Free Research Field

整形外科