2017 Fiscal Year Final Research Report
The role of Cholecystokinin B receptor (CCKBR) in axonal elongation
| Project/Area Number |
15K10397
|
|---|
| Research Category |
Grant-in-Aid for Scientific Research (C)
|
| Allocation Type | Multi-year Fund |
|---|
| Section | 一般 |
|---|
| Research Field |
Orthopaedic surgery
|
|---|
| Research Institution | Hamamatsu University School of Medicine |
|---|
Principal Investigator |
|
|---|
| Project Period (FY) |
2015-04-01 – 2018-03-31
|
| Keywords | conditioning / nerve regeneration / cckbr / dorsal root ganglion |
|---|
| Outline of Final Research Achievements |
Pre-conditioning peripheral nervous system (PNS) neurons by a peripheral axonal injury primes them through massive transcriptional changes to regenerate more vigorously and faster in the face of a second injury because of an induction of networks of regeneration-associated genes. Studies of the transcriptional response in pre-conditioned dorsal root ganglion (DRG) neurons have implicated regeneration-promoting roles for many transcription factors and regeneration-associated genes. Among them, cholecystokinin B receptor (CCKBR) gene increases ten times at DRG after pre-conditioning. However, the role of CCKBR in regeneration is yet to be elucidated. The purpose of this study is to investigate the role of CCKBR in association with peripheral nerve regeneration. In loss-of-function experiments, LY225910 significantly reduced neurite initiation (53% reduction, P＜0.001) and maximal axon length (64% reduction, P＜0.001) relative to vehicle controls.
|
| Free Research Field |
神経再生
|
