CDK4 target therapy for bone and soft tissue tumors

2017 Fiscal Year Final Research Report

• Project/Area Number: 15K10434
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Orthopaedic surgery
• Research Institution: Niigata University
• Principal Investigator: Ogose Akira 新潟大学, 医歯学総合病院, 特任教授 (80323963)
• Project Period (FY): 2015-04-01 – 2018-03-31
• Keywords: liposarcoma / CDK4 / osteosarcoma

Outline of Final Research Achievements

Expression of mRNA of MDM2, CDK4 of bone soft tissue sarcoma of 148 cases was evaluated by quantitative PCR. MDM 2, CDK 4, tumors that are more than twice as much expressed as normal fats are well-differentiated liposarcoma 32/35 cases, and dedifferentiated liposarcoma 5/5 cases, which were highly specific to these two types of liposarcoma. In other words, high tumors with both factors are diagnosed at first glance to other tissue typesGrowth inhibition tests were performed in vitro using two CDK4 inhibitors (LY 2835219, PD 0332991). The IC50 for liposarcoma cell NDDLS1 was LY2835219 at 19.5 μmol / L. The original hypothesis that inhibitors act specifically on liposarcoma highly expressing CDK4 was denied. this is. It is presumed that in NDDLS1 cells, only CDK4 is an experimental result showing the possibility that it is not a factor regulating proliferation.

Free Research Field

整形外科学