Novel strategy for bone regeneration using micro RNA

2017 Fiscal Year Final Research Report

• Project/Area Number: 15K10443
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Orthopaedic surgery
• Research Institution: Kobe University
• Principal Investigator: Oe Keisuke 神戸大学, 医学部附属病院, 助教 (20514623)
• Co-Investigator(Kenkyū-buntansha): 新倉 隆宏 神戸大学, 医学研究科, 講師 (40448171) ; 李 相亮 昭和大学, 医学部, 講師 (40533732) ; 岩倉 崇 神戸大学, 医学部附属病院, その他 (60437473) ; 福井 友章 神戸大学, 医学部附属病院, 医員 (50437688)
• Project Period (FY): 2015-04-01 – 2018-03-31
• Keywords: microRNA / 骨再生 / 骨折 / 偽関節 / 血管新生

Outline of Final Research Achievements

After creating a closed femoral fracture model in rats, miRNA was extracted from the newly generated tissue at the fracture site. Microarray and real-time PCR analyses were performed with miRNA samples. From the results, we selected two angionenesis-related miRNAs, miR-126a-3p and miR-146a-5p. We next investigated whether local administration of anti-miRNA oligonucleotides of miR-126a-3p or miR-146a-5p into the fracture site could achieve bone union in a rat refractory femur fracture model. Radilogical and histological assessments revealed that these treatments did not lead to successful bone union at week 8.

Free Research Field

整形外科学