The influence of gelatin hydrogel including eicosapentaenoic acid on the osteoarthritis progression in vivo.

2017 Fiscal Year Final Research Report

• Project/Area Number: 15K10472
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Orthopaedic surgery
• Research Institution: Kobe University
• Principal Investigator: Hayashi Shinya 神戸大学, 医学部附属病院, 助教 (20437487)
• Project Period (FY): 2015-04-01 – 2018-03-31
• Keywords: EPA / 軟骨 / 変形性関節症

Outline of Final Research Achievements

Eicosapentaenoic acid (EPA) is an antioxidant and omega-3 (n-3) polyunsaturated fatty acid that reduces the production of inflammatory cytokines. We previously reported that EPA treatment inhibited chondrocyte apoptosis and MMP expression induced by oxidative stress in vitro, and prevented osteoarthtitis (OA) progression in vivo.The purpose of this study is to investigate the effect of gelatin hydrogel including EPA on the OA progression in vivo.Thirty-six male C57BL/6J mice were randomly divided into 6 groups (n = 6 mice each): sham, DMM, DMM injected with corn oil, DMM injected with corn oil and EPA, DMM with control gelatin hydrogel and DMM with gelatin hydrogel including EPA. Corn oil or corn oil with EPA was injected once into the knee joint at timing of DMM surgery.we investigated the effect of gelatin hydrogel including EPA on the OA progression in vivo. The gelatin hydrogel including EPA prevented OA progression more effective than single injection of EPA in vivo.

Free Research Field

整形外科学