Attempt to establish chimeric osteoclast co-culture system and mechanistic analysis of osteoclast differentiation

2017 Fiscal Year Final Research Report

• Project/Area Number: 15K10488
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Orthopaedic surgery
• Research Institution: Saitama Medical University
• Principal Investigator: SATO Kojiro 埼玉医科大学, 医学部, 准教授 (10372434)
• Research Collaborator: YANAGISAWA Maiko ; TACHIBANA Hideyuki ; AIZAKI Yoshimi ; YAZAWA Hiroaki ; ARAKI Yasuto ; MIMURA Toshihide
• Project Period (FY): 2015-04-01 – 2018-03-31
• Keywords: 破骨細胞 / 骨芽細胞 / 滑膜由来線維芽細胞 / 破骨細胞分化因子

Outline of Final Research Achievements

Synovial fibroblasts cannot be substituted for osteoblasts in osteoclast differentiation. They apparently do not provide sufficient survival factor(s). They also do not provide enough RANKL. Instead, they produce a large amount of OPG. Thus, osteoclasts observed in the pannus may be dependent on RANKL from other sources, or may be differentiated by stimulation of cytokines other than RANKL, such as TNF-α and IL-6. Mouse osteoblasts also produce a large amount of OPG, which may function as a mechanism for preventing ectopic osteoclastogenesis.

Free Research Field

免疫学