Role of Heparan Sulfate 6-O endosulfatase and establishment of the novel meniscal scaffold

2017 Fiscal Year Final Research Report

• Project/Area Number: 15K10498
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Orthopaedic surgery
• Research Institution: Osaka Medical College
• Principal Investigator: Otsuki Shuhei 大阪医科大学, 医学部, 講師 (20589840)
• Co-Investigator(Kenkyū-buntansha): 根尾 昌志 大阪医科大学, 医学部, 教授 (80311736)
• Research Collaborator: NAKAGAWA Kosuke 大阪医科大学, 医学研究科, 大学院生
• Project Period (FY): 2015-04-01 – 2018-03-31
• Keywords: 半月板 / へパラン硫酸 / シート状scaffold

Outline of Final Research Achievements

Meniscal injury is treated with suture or partial meniscectomy in Japan, however irreparable injury has still to be investigated for the novel surgical technique because it goes osteoarthritic changes dramatically. Heparansulfate 6-O endosulfatases (Sulfs) had reported to be the essential factor for cartilage homeostasis and therapeutic potential for OA. In this research, We investigated the role of Sulf-1 in meniscus by using KO mice and its mechanism. As the result, Sulf-1 was associated with the meniscus homeostasis and type II collagen expression was reduced in Sulf-1 KO menisci. TGF/Smad cell signaling was accelerated with Sulf-1 supplementation, which overexpressed type II collagen. As the next step, novel meniscus therapy by using scaffold has been developed. We Scaffold involving Sulf-1 function may have a therapeutic potential in the near future.

Free Research Field

膝、半月板、軟骨