Lidocaine binding assay to investigate the toxicity of lidocaine

2018 Fiscal Year Final Research Report

• Project/Area Number: 15K10508
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Anesthesiology
• Research Institution: Shinshu University
• Principal Investigator: Ishida Takashi 信州大学, 学術研究院医学系(医学部附属病院), 講師 (60531952)
• Project Period (FY): 2015-04-01 – 2019-03-31
• Keywords: 局所麻酔薬 / 毒性

Outline of Final Research Achievements

We investigated the protein binding site of lidocaine to elucidate the target of the lidocaine toxicity. Lidocaine and FG beads were immobilized for using as probe of lidocaine binding protein. Lysate of Hela cells was mixed with the probe and the fractions were corrected to western blotting for detection of the receptor. Several bands were detected at 40 kDa, 50 kDa, and 60 kDa in the wester blotting. However, affinity of the lidocaine binding probe was very low and the specific binding could not be detected.

Free Research Field

麻酔科学

Academic Significance and Societal Importance of the Research Achievements

本研究は、リドカインの毒性のメカニズムを明らかとすることを目的として研究を行った。リドカイン結合磁性ビーズを用いてリドカインの結合するタンパクを探索した。この研究により、数種類のタンパクがリドカインに結合することが明らかとなった。これらのタンパクのうち特異的に結合するものを調べたが、特異的に結合するタンパクを見つけるには至らなかった。