Elucidation of the mechanism of action of volatile anesthetics by visualization of membrane microdomains and receptors

2017 Fiscal Year Final Research Report

• Project/Area Number: 15K10514
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Anesthesiology
• Research Institution: Kagawa University
• Principal Investigator: Ono Junichiro 香川大学, 医学部, 協力研究員 (90363217)
• Co-Investigator(Kenkyū-buntansha): 樺山 一哉 大阪大学, 理学研究科, 准教授 (00399974)
• Co-Investigator(Renkei-kenkyūsha): SUZUKI Shingo 香川大学, 医学部, 准教授 (50451430)
• Project Period (FY): 2015-04-01 – 2018-03-31
• Keywords: 揮発性麻酔薬 / 作用機序 / ライブセルイメージング / FRAP解析 / マイクロドメイン / 膜流動性 / シグナル伝達

Outline of Final Research Achievements

We studied the mechanism of action of volatile anesthetics using live cell imaging. Especially, fluorescence recovery after photobleaching (FRAP) enables characterization of quantitative dynamic properties in living cells. We visualized membrane microdomains and receptors using HaloTag fusion proteins for the FRAP analysis. The fluidity of HaloTag fused transferrin receptors, a marker of the non-microdomain fraction, was augmented by isoflurane exposure. Whereas, no significant difference was detected in the HaloTag fused GPI anchor proteins. Since lateral diffusion of transmembrane proteins such as transferrin receptors influences signal transduction, it is considered that enhanced lateral diffusion by volatile anesthetics may contribute to the mechanism of action.

Free Research Field

麻酔科学