2017 Fiscal Year Final Research Report
In vitro study of the functinal significane of ischemic tolerance-induced changes in the expression level of aconitase2 and aldolase A
| Project/Area Number |
15K10520
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Anesthesiology
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| Research Institution | Osaka Prefecture University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
竹中 重雄 大阪府立大学, 総合リハビリテーション学研究科, 教授 (10280067)
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| Project Period (FY) |
2015-04-01 – 2018-03-31
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| Keywords | 脳神経疾患 / 脳虚血 / 海馬 / 虚血耐性 |
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| Outline of Final Research Achievements |
This study aimed to examine whether aconitase 2, an enzyme of tricarboxylic acid cycle, and aldolase A, an enzyme of glycolytic system, are key proteins for protecting neurons from ischemic injury using a cultured fetal hippocampal neurons. In this study, in vitro model of ischemia were mimicked by oxygen-glucose deprivation (OGD). OGD-induced cell death was assessed by measuring the activity of lactate dehydrogenase (LDH) that leaked into the culture medium. OGD for 180 min induced significantly neuronal cell death. The decrease in expression level of aconitase 2 mRNA and protein after ransfection of aconitase 2 siRNA were confirmed by RT-PCR and western blot, respectively. There was not significant difference in OGD-induced neuronal cell death between aconitase 2 siRNA transfected neurons and control siRNA transfected neurons.
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| Free Research Field |
中枢神経
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