The influence of anesthetic agent on the plasty of neuronal circuit in the developmental brain

2017 Fiscal Year Final Research Report

• Project/Area Number: 15K10528
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Anesthesiology
• Research Institution: National Defense Medical College
• Principal Investigator: Kodama Mitsuyoshi 防衛医科大学校(医学教育部医学科進学課程及び専門課程、動物実験施設、共同利用研究施設、病院並びに防衛, 病院 麻酔科, 講師 (00597528)
• Project Period (FY): 2015-04-01 – 2018-03-31
• Keywords: 麻酔薬 / 発達期脳神経 / 毒性

Outline of Final Research Achievements

Propofol increased RhoA activation, collapsed growth cones, and impaired retrograde axonal transport ofquantum dot-labelled brain-derived neurotrophic factor, all of which were prevented with TAT-C3. Adult mice previously treated with propofol had decreased numbers of total hippocampal synapses and presynaptic vesicles, reduced hippocampal dendritic arborisation, and infrapyramidal mossy fibres. These mice also exhibited decreased hippocampaldependent contextual fear memory recall. All anatomical and behavioural changes were prevented with TAT-C3 pretreatment.

Free Research Field

麻酔学