Development of a new treatment strategy to target mitochondrial chaperones in urothelial carcinoma

2017 Fiscal Year Final Research Report

• Project/Area Number: 15K10579
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Urology
• Research Institution: Tokyo Medical and Dental University
• Principal Investigator: YOSHIDA Soichiro 東京医科歯科大学, 大学院医歯学総合研究科, 助教 (80383280)
• Co-Investigator(Kenkyū-buntansha): 小野 竜一 国立医薬品食品衛生研究所, 毒性部, 室長 (10401358) ; 齋藤 一隆 東京医科歯科大学, 医歯(薬)学総合研究科, 助教 (10422495) ; 藤井 靖久 東京医科歯科大学, 医歯(薬)学総合研究科, 准教授 (70282754)
• Project Period (FY): 2015-04-01 – 2018-03-31
• Keywords: 分子シャペロン / ミトコンドリア

Outline of Final Research Achievements

TRAP1 (TNF receptor-associated protein), a homologue of HSP90, is mitochondrial molecular chaperone which regulates a metabolic switch between mitochondrial respiration and aerobic glycolysis. We explored the possibility that TRAP1 phosphorylation regulates its chaperone activity. We identified Y498 as a possible tyrosine phosphorylated residue, and found attenuation of c-Src mediated phosphorylation in TRAP1 with non-phosphomimetic mutation of this residue, while there was no significant difference in the ATP binding activity between the wild type, and phospho- or non-phosphomimetic mutation TRAP1.

Free Research Field

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