2017 Fiscal Year Final Research Report
Functional analysis of the prostate cancer-related genes obtained from the new tissue culture system
| Project/Area Number |
15K10580
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Urology
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| Research Institution | Mie University (2017)
Yokohama National University (2015-2016) |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
白石 泰三 三重大学, 医学系研究科, 客員教授 (30162762)
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| Project Period (FY) |
2015-04-01 – 2018-03-31
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| Keywords | 前立腺癌 / ３次元培養 / 抗癌剤 |
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| Outline of Final Research Achievements |
In our previous study, the prostate cancer-related genes were identified in the new tissue culture system. Among these genes showing enhanced expression in the new tissue culture system, the KIF22 gene was extracted as one of candidate genes related with prostate cancer behavior. In this study, we analyzed proliferation, apoptosis, and cell cycle of the prostate cancer cells (DU-145 and LNCaP) treated with KIF22 siRNA. The inhibition of KIF22 mRNA using siRNA affected cell proliferation in spheroids and monolayers of both cancer cell lines. This suppression also caused G2/M arrest and apoptosis in both cell lines. These results suggest involvement of the KIF22 gene in prostate cancer behavior.
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| Free Research Field |
腫瘍病理、ナノメディシン
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