Functional bladder regeneration with tissue engineering urothelium using human amnion: a study on dogs

2017 Fiscal Year Final Research Report

• Project/Area Number: 15K10623
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Urology
• Research Institution: Shiga University of Medical Science
• Principal Investigator: Johnin Kazuyoshi 滋賀医科大学, 医学部, 講師 (90324590)
• Co-Investigator(Kenkyū-buntansha): 小林 憲市 滋賀医科大学, 医学部, 助教 (40727434) ; 影山 進 滋賀医科大学, 医学部, 講師 (50378452) ; 河内 明宏 滋賀医科大学, 医学部, 教授 (90240952)
• Co-Investigator(Renkei-kenkyūsha): Akeo Hagiwara 同志社大学, 生命医科学部, 教授 (90198648)
• Project Period (FY): 2015-04-01 – 2018-03-31
• Keywords: 膀胱再生

Outline of Final Research Achievements

The aim is to regenerate an autologous functional bladder tissue using amnion sheets and bioabsorbable scaffold with poly-lactic acid and poly-caprolactone copolymer P (LS/CL).
We cultured cells from oral buccal mucosa and stomach smooth muscle cells on the amnion and P(LS/CL) sheets. Moreover, epithelium (amnion sheet), middle layer(omentum), and muscle layer P(LS/CL) sheets were piled on subsequently, and wrapped by omentum for 3 weeks. Immunohistochemical studies showed that the epithelium was positive for cytokeratin and muscle layer was positive for α-SMA. Next we augmented regenerative bladder tissue onto native bladder with sufficient blood supply from omentum according to Clam method. After 6 months observation there were no complications, cystoscopic finding and cystography showed normal shaped bladder with normal peristalsis. These results indicated successful regeneration of functional bladder tissue using autologous tissues

Free Research Field

泌尿器科学