Effect of Octanoic acid and lipid metabolism in preembryo and pluripotent stem cells culture

2017 Fiscal Year Final Research Report

• Project/Area Number: 15K10692
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Obstetrics and gynecology
• Research Institution: Tokyo Medical University
• Principal Investigator: KUJI NAOAKI 東京医科大学, 医学部, 教授 (80169987)
• Co-Investigator(Kenkyū-buntansha): 伊東 宏絵 東京医科大学, 医学部, 講師 (00307307) ; 井坂 恵一 東京医科大学, 医学部, 教授 (10201310) ; 阿久津 英憲 国立研究開発法人国立成育医療研究センター, 生殖医療研究部, 部長 (50347225) ; 小田 佳奈子 新潟大学, 脳研究所, 助教 (60708212)
• Project Period (FY): 2015-04-01 – 2018-03-31
• Keywords: マウス / 体外受精 / オクタン酸 / 胚発生 / インプリンティング

Outline of Final Research Achievements

It was demonstrated that commercially available human in-vitro fertilization medium had contained 500uM-1000uM octanoic acid, using mass spectrometric analysis. Using F1 and inbred mice in-vitro fertilized embryo, several effects were recognized in the medium containing 800 and 1200uM of OA ; 1) in vitro embryo development (up to blastocyst stage) were delayed and disturbed, 2) implantation rates were decreased and 3) body weight were decreased after 179days after birth. Gene expression analysis revealed that expression pattern were different in several genes, including imprinting genes.

Free Research Field

産婦人科学（生殖医学）