2017 Fiscal Year Final Research Report
Redox regulation of estrogen and miRNA-222 in adipocytes
| Project/Area Number |
15K10700
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Obstetrics and gynecology
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| Research Institution | Yamagata University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
山谷 日鶴 山形大学, 医学部, 助教 (40550637)
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| Project Period (FY) |
2015-04-01 – 2018-03-31
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| Keywords | エストロゲン / 閉経 / 内臓脂肪 / 酸化ストレス |
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| Outline of Final Research Achievements |
The size of visceral adipocytes in postmenopausal women was significantly larger than that in premenopausal women (P < 0.05). The lipid peroxidation levels of visceral fat in postmenopal women were also significantly higher than that in premenopausal women (P < 0.05). We found that oxidative stress in visceral fat is higher in postmenopausal women. Estradiol (E2) significantly reduced the intracellular ROS levels that were induced by H2O2 in 3T3-L1 adipocytes (P < 0.01). We determined that E2 significantly increased the expression of nuclear factor erythroid 2-related factor 2 (NRF2)-dependent antioxidant genes, heme oxygenase-1 (HO-1), NAD(P)H:quinone oxidoreductase 1 (NQO-1), and the glutamate-cysteine ligase modifier subunit (GCL) genes in 3T3-L1 adipocytes (P < 0.01). Hence, estrogen may act as an antioxidant in the adipose tissues of premenopausal women.
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| Free Research Field |
医歯薬学
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