Genome-wide DNA methylation analysis in endometrial cancer

2017 Fiscal Year Final Research Report

• Project/Area Number: 15K10727
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Obstetrics and gynecology
• Research Institution: Keio University
• Principal Investigator: YANOKURA Megumi 慶應義塾大学, 医学部(信濃町), 特任助教 (20433732)
• Co-Investigator(Kenkyū-buntansha): 阪埜 浩司 慶應義塾大学, 医学部(信濃町), 准教授 (70265875)
• Project Period (FY): 2015-04-01 – 2018-03-31
• Keywords: epimutation / 子宮体癌 / miR-663a / CIMP

Outline of Final Research Achievements

[Objective] Concurrent DNA methylation of multiple genes occurs in endometrial cancer. However, the features and causes of high-methylation rate endometrial cancer are not well understood. Therefore, the aim of this study was to investigate the DNA methylation status in normal tissue from patients with endometrial cancer.
[Methods] The study was approved by the Ethics Committee. The subjects were 25 patients with endometrial cancer from whom cancer tissue and peripheral blood cells (PBCs) were obtained. The 25 cancer samples were classified as Methyl-high, and Methyl-negative (Methyl(-)) by methylation-specific PCR (MSP) analysis of three genes. DNA libraries were prepared from peripheral blood DNAs from 2 Methyl-H and 2 Methyl(-) patients, and bisulfite sequencing was performed.
[Results] PBC DNA in Methyl-H cases had significant hypermethylation in the miR-663a promoter region, compared to Methyl(-) cases.

Free Research Field

婦人科腫瘍学