Identification of endometrial adenocarcinoma biomarkers and therapeutic target candidates by proteomic analysis

2018 Fiscal Year Final Research Report

• Project/Area Number: 15K10735
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Obstetrics and gynecology
• Research Institution: Nippon Medical School
• Principal Investigator: Peng Wei-Xia 日本医科大学, 医学部, 講師 (00535700)
• Co-Investigator(Kenkyū-buntansha): 内藤 善哉 日本医科大学, 大学院医学研究科, 大学院教授 (20237184) ; 和田 龍一 日本医科大学, 医学部, 准教授 (20260408)
• Project Period (FY): 2015-04-01 – 2019-03-31
• Keywords: 子宮内膜癌

Outline of Final Research Achievements

To identify potential prognostic biomarkers in endometrioid carcinoma (EC), we performed proteomic analysis and immunohistochemistry (IHC). Firstly, we revealed the total protein expression in 3 grades ECs respectively, and compared the protein expression profiles between grades. Then we validated the protein expression using IHC and compared the protein expression patterns with clinical factors. Compare to G1 EC, differentially expressed proteins in G3 was 56 types. Among them, we focused the Filamin A. In IHC study, G3 ECs showed significantly higher expression of Filamin A than G1 and G2 ECs. Furthermore, strong expression of Filamin A tended to be associated with cancer frequent recurrence and lymphovascular invasion.The current analysis revealed the significant association of high expression Filamin A and unfavorable prognostic factors of ECs, suggesting that Filamin A may play a critical role in EC invasiveness and prognosis.

Free Research Field

腫瘍

Academic Significance and Societal Importance of the Research Achievements

今回の検討では、Filamin Aの発現は子宮類内膜癌の脱分化に関与する可能性が示唆され、予後不良のバイオマーカーとして有用の可能性が考えられる。