2017 Fiscal Year Final Research Report
Establishment of quantitative methods to evaluate mitochondrial function in the inner ear
| Project/Area Number |
15K10743
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Otorhinolaryngology
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| Research Institution | The University of Tokyo |
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Principal Investigator |
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| Research Collaborator |
KINOSHITA MAKOTO
KAMOGASHIRA TERU
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| Project Period (FY) |
2015-04-01 – 2018-03-31
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| Keywords | 内耳 / ミトコンドリア / 加齢 / 有毛細胞 / 蝸牛 / 電子伝達系 / ミトコンドリア膜電位 / 酸化ストレス |
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| Outline of Final Research Achievements |
We established quantitative method to evaluate mitochondrial membrane potential by using JC-1 fluorescence dye in HEI-OC1 auditory cell line and cochlear explants. We also established quantitative method to evaluate mitochondrial respiratory function by using an extracellular flux analyzer in HEI-OC1 auditory cell line and cochlear explants.
We then evaluated mitochondrial function of a senescence model of HEI-OC1 cells induced by H2O2 exposure, by these established quantitative methods.
We revealed that relative value of oxygen consumption rate on FCCP injection was dose dependently decreased in the H2O2-exposed cells. This suggests damage of the mitochondrial respiratory capacity in the senescence model.We also revealed that JC-1 red/green ratio was decreased in a dose-dependent manner in the H2O2-exposed cells. This suggests decreased mitochondrial membrane potential in the senescence model.
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| Free Research Field |
耳鼻咽喉科学
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