Basic research to investigate the mechanism of vestibuar migraine

2017 Fiscal Year Final Research Report

• Project/Area Number: 15K10772
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Otorhinolaryngology
• Research Institution: 独立行政法人国立病院機構(東京医療センター臨床研究センター)
• Principal Investigator: Goto Fumiyuki 独立行政法人国立病院機構(東京医療センター臨床研究センター), その他部局等, 医師 (80286567)
• Co-Investigator(Kenkyū-buntansha): 北原 糺 奈良県立医科大学, 医学部, 教授 (30343255) ; 鳥海 春樹 慶應義塾大学, 医学部(信濃町), 講師(非常勤) (30528203)
• Project Period (FY): 2015-04-01 – 2018-03-31
• Keywords: 片頭痛 / 細胞死 / 疼痛刺激 / TRPA1 / エクソソーム / TLR7 / microRNA let-7b

Outline of Final Research Achievements

MiRNA let-7b play a major role to regulate cell death through the interaction with Toll-like Receptor 7 (TLR7). The number of apoptotic cells were significantly increased in let-7b-exposed cells. The expression of TLR7 was increased in let-7b-exposed cells. However, the number of apoptotic cells remained unchanged in let-7b-exposed TLR7 knock down cells. The number of apoptotic cells remained unchanged in let-7b inhibitor-treated cells after exposed to TLR7 agonist. The expression of creaved-caspase-3 and LC3-II was significantly increased in TLR7 agonist -exposed cells, while let-7b inhibitor regulated apoptosis mediated by TLR7 agonist. Our results indicated that miRNA let-7b/TLR7 signaling interaction could be a new therapeutic target for auditory cell death.

Free Research Field

医学