Establishment of selective cell transplantation for specific differentiation into olfactory sensory neurons

2019 Fiscal Year Final Research Report

• Project/Area Number: 15K10783
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Otorhinolaryngology
• Research Institution: Okayama University
• Principal Investigator: Takahara Junko 岡山大学, 医学部, 技術専門職員 (80448224)
• Co-Investigator(Kenkyū-buntansha): 西崎 和則 岡山大学, 医歯薬学総合研究科, 教授 (90180603)
• Project Period (FY): 2015-04-01 – 2020-03-31
• Keywords: 細胞・組織

Outline of Final Research Achievements

In this study, we investigated a method for isolating and culturing BMSCs (bone marrow-derived mesenchymal stem cells) for the purpose of improving the rate of differentiation into olfactory sensory neurons by transplanting one type of cells.
In cell isolation using a flow cytometer, loss of cells was large due to immaturity of technical skills. Therefore, it was attempted to remove unnecessary cells in advance and increase the frequency of the target cells by using immunomagnetic cell separation.The immunomagnetic cell separation used in this study had the advantage of being relatively simple and easy to introduce. This method was performed after collecting the cells from the bones, but proliferation of mesenchymal stem cells could not be confirmed.Therefore, it was considered necessary to increase the number of times of this method to improve the purity.

Free Research Field

鼻科学

Academic Significance and Societal Importance of the Research Achievements

様々な細胞が存在する集団から存在頻度の非常に少ない細胞をソーティングするには、
熟練した技術力が必要であり、習得に多くの時間を要する。また、実験者によるデータの差も大きくなる。そこで本研究で得られた成果は、存在頻度の非常に少ない細胞をソーティングする際に生じる技術力等の問題を解決する上で有用な情報になると考えられる。