2017 Fiscal Year Final Research Report
Leukotriene E4 receptor in human nasal mucosa-the role of GPR99 and the clinical application for upper airway diseases.
| Project/Area Number |
15K10786
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Otorhinolaryngology
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| Research Institution | Sapporo Medical University |
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Principal Investigator |
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| Project Period (FY) |
2015-04-01 – 2018-03-31
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| Keywords | ロイコトリエンE4 / GPR99 / P2Y12 / 鼻粘膜 / 血管内皮細胞 |
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| Outline of Final Research Achievements |
The cysteinyl leukotrienes (CysLTs) are lipid mediators that have been implicated in the pathogenesis of allergic rhinitis. CysLT1 receptor is sensitive to the CysLT1 receptor antagonist currently used to treat allergic rhinitis and asthma. Recent studies have begun to uncover receptors selective for LTE4: GPR99. To clarify the expression of GPR99 receptor in human nasal mucosa, we investigated immunohistochemical localization of GPR99 receptor in human nasal mucosa, and intracellular calcium influx in vitro. The immunohistochemical study revealed that vascular smooth muscle and vascular endothelial cells showed intense immunoreactivity for GPR99 receptor. LTE4 caused a rapid increase of the intracellular Ca2+ concentration. Both CysLT1 and CysLT2 receptor antagonists did not affect the LTE4-induced intracellular Ca2+ response. These results suggest that a potential third CysLT receptor such as GPR80/99 receptor may contribute LTE4-induced activation of nasal vascular cells.
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| Free Research Field |
鼻アレルギー
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