Analysis on pathological condition control of corio-retinal diseases using human polarized retinal pigment epithelial cells

2017 Fiscal Year Final Research Report

• Project/Area Number: 15K10873
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Ophthalmology
• Research Institution: Kagoshima University
• Principal Investigator: SONODA Shozo 鹿児島大学, 医歯学域附属病院, 講師 (20325806)
• Co-Investigator(Kenkyū-buntansha): 大塚 寛樹 鹿児島大学, 医学部・歯学部附属病院, その他 (20611817)
• Project Period (FY): 2015-04-01 – 2018-03-31
• Keywords: 網膜色素上皮細胞 / 極性RPE細胞 / 抗VEGF抗体 / 脈絡膜 / イメージング

Outline of Final Research Achievements

Our group had established a stable culture method of polarized RPE by using the human commercial available RPE cell lines on Transwell. We had investigated drug permeability of anti-VEGF antibody which were currently used clinically with our polarized RPE, and found that the permeability ratio differs depending on the drug. We also analyzed the difference in response by TNF-α stimulation with or without polarity and found the ability of VEGF secretion and the interaction of intracellular signal were different depending on the polarity.
In the image analysis, there are structural changes of the choroid in eyes with CSC, the larger hyperreflective area change in the inner choroid and the larger hyporeflective area change in the outer choroid by using binarization methods. Most importantly, the quantification of the structural components of the choroid in the OCT B-scan images has extracted new information which should contribute to the determination of the mechanism of CSC.

Free Research Field

眼科学