2017 Fiscal Year Final Research Report
Spheroidal cultivation of the human limbal epithelial niche from small limbal tissue.
| Project/Area Number |
15K10907
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Ophthalmology
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| Research Institution | Tokyo Dental College |
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Principal Investigator |
HIGA KAZUNARI 東京歯科大学, 歯学部, 助教 (60398782)
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| Project Period (FY) |
2015-04-01 – 2018-03-31
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| Keywords | corneal limbal niche |
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| Outline of Final Research Achievements |
To improve the cultivation method, we assumed a limited autologous tissue and performed spheroidal cultivation from human small limbal tissue. Uniformly small and round spheroids were formed even from small limbal tissue. Efficiency of spheroidal formation was differed in the derived site of the original limbus. Spheroids derived from the high spheroidal formation site showed the large colony forming efficiency and higher ratio of slow cycling cells. In addition, N-cadherin was infrequently-expressed in epithelial spheroid. Spheroids were maintained post-operative adaptation and epithelial proliferation in a limbal-deficient rabbit transplantation model.
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| Free Research Field |
眼科学
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