Elucidation of the mechanism of the sensory recovery in the denervated skin flap -Attention to a signal from keratinocyte -

2017 Fiscal Year Final Research Report

• Project/Area Number: 15K10937
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Plastic surgery
• Research Institution: Tokyo Medical and Dental University
• Principal Investigator: MORI Hiroki 東京医科歯科大学, 大学院医歯学総合研究科, 講師 (80345305)
• Co-Investigator(Kenkyū-buntansha): 岡崎 睦 東京大学, 医学部附属病院, 助手 (50311618)
• Project Period (FY): 2015-04-01 – 2018-03-31
• Keywords: 無知覚皮弁 / 神経再生 / 共培養 / 角化細胞 / 神経細胞

Outline of Final Research Achievements

TRPV1 (VR1), P2X3, nerve growth factor (NGF), and semaphorin were detected in denervated skin flap. TRPV1, and NGF tended to increase in the dermis of denervated skin, and semaphorin decreased there. I established cocultivation model of keratinocyte and dorsal-root-ganglion-derived cells. Contact coculture and non-contact coculture were compared by NGF, and BDNF Elisa. NGF in contact group tended to increase.

Free Research Field

形成外科学