Spontaneously Generated Large Adipose Flap in an in vivo Tissue-Engineering Chamber

2017 Fiscal Year Final Research Report

• Project/Area Number: 15K10940
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Plastic surgery
• Research Institution: Kagawa University
• Principal Investigator: Hamamoto Yusuke 香川大学, 医学部附属病院, 助教 (10380180)
• Co-Investigator(Renkei-kenkyūsha): UENO Masaki 香川大学, 医学部炎症病理, 教授 (30322267) ; TAMAI Motogi 香川大学, 医学部形成外科, 助教 (90617276) ; KOGURE Tetsukuni 香川大学, 医学部形成外科, 助教 (40437695)
• Research Collaborator: TANAKA Yoshio 香川大学, 医学部形成外科, 研究協力員 (50171806)
• Project Period (FY): 2015-04-01 – 2018-03-31
• Keywords: 再生医療 / 脂肪組織 / PRP / bFGF / chamber / 線維芽細胞様細胞

Outline of Final Research Achievements

The present study clarified 1) the reproducibility of creating an adipose flap, 2) the time-course of adipogenesis, 3) the long-term stability of a generated adipose flap, and 4) the mechanism of adipogenesis in an in vivo chamber. The chamber was used as a protected space for tissue engineering, the vascular pedicle as a vascular source, the collagen sponge as a scaffold, and PRP and c-bFGF as growth factors.Results)The ratios of the vascular pedicle and adipose tissue in the generated tissue were 14.8%, 47% and 80% at 4, 8, and 12　weeks, respectively. The adipose flap in Group 12w maintained its volume and shape for five months following pedicle transfer. Conclusions:Adipose flaps were spontaneously generated at 12 weeks with reproducibility, and showed long-term stability. Fibroblast-like cells recruited from the vascular pedicle was thought to contribute to the adipogenesis in the tissue-engineering chamber.

Free Research Field

形成外科