Establishment of methods for immune response analysis of lymphocytes and neutrophils against sepsis and trauma

2017 Fiscal Year Final Research Report

• Project/Area Number: 15K10979
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Emergency medicine
• Research Institution: Osaka University
• Principal Investigator: Shimizu Kentaro 大阪大学, 医学部附属病院, 助教 (60379203)
• Co-Investigator(Kenkyū-buntansha): 高橋 弘毅 関西医科大学, 医学部, 助教 (30609590) ; 吉矢 和久 大阪大学, 医学部附属病院, 助教 (40379201) ; 嶋津 岳士 大阪大学, 医学系研究科, 教授 (50196474) ; 池田 光憲 大阪大学, 医学部附属病院, 医員 (60548444) ; 小倉 裕司 大阪大学, 医学系研究科, 准教授 (70301265) ; 松本 寿健 大阪大学, 医学系研究科, 招へい教員 (70644003)
• Project Period (FY): 2015-04-01 – 2018-03-31
• Keywords: 免疫 / インフラマソーム / T細胞 / 痰 / 保存 / フローサイトメトリー / 全身性炎症反応

Outline of Final Research Achievements

Immune system occurred inflammatory response against host in respond to not only pathogenic organisms but also self tissues following trauma. Evaluation and modulation of this inflammatory response is critical for treatments of critically illnesses. In this research, we investigated following results; 1. A preservation method of immune cells was developed. 2. Inflammasome, which is one of the danger signals, was activated following injury. CD8+Tcells were decreased and CD4+Tcells and Bcells were increased. 3. Immune cells from aspirated sputum mainly consisted of neutrophils. There were few lymphocytes. 4. Immune response in the intestine might be different with gut microbiota.

Free Research Field

救急医学・集中治療医学