The functional study of GABA and serotonin in differentiation of taste bud cells using Cre-loxP system

2017 Fiscal Year Final Research Report

• Project/Area Number: 15K11018
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Morphological basic dentistry
• Research Institution: Kyushu Dental College
• Principal Investigator: Seta Yuji 九州歯科大学, 歯学部, 教授 (90291616)
• Co-Investigator(Kenkyū-buntansha): 豊野 孝 九州歯科大学, 歯学部, 准教授 (10311929) ; 中富 満城 九州歯科大学, 歯学部, 講師 (10571771) ; 小野 堅太郎 九州歯科大学, 歯学部, 教授 (40316154) ; 片岡 真司 九州歯科大学, 歯学部, 助教 (80364149)
• Project Period (FY): 2015-04-01 – 2018-03-31
• Keywords: 味蕾 / 転写因子 / Mash1 / セロトニン / GABA

Outline of Final Research Achievements

In the present study, to begin to understand these mechanisms, we investigated the role of Mash1-positive cells in regulating adult taste bud cell differentiation through the loss of Mash1-positive cells using the Cre-loxP system. We found that the cells expressing type III cell markers; aromatic L-amino acid decarboxylase (AADC), carbonic anhydrase 4 (CA4), glutamate decarboxylase 67 (GAD67), neural cell adhesion molecule (NCAM), and synaptosomal-associated protein 25 (SNAP25); were significantly reduced in the circumvallate taste buds after administration of tamoxifen. However gustducin and Phospholipase C beta2 (PLC beta2);markers of type II taste bud cells; were not significantly changed in the circumvallate taste buds after administration of tamoxifen. These results suggest that Mash1-positive cells could be differentiated to the type III cells, not to the type II cells in the taste bud.

Free Research Field

解剖学